Tristan Thompson sent a cease-and-desist letter to the podcast which aired an interview with an Instagram model claiming the sports star bedded her.
Fresh cheating claims have rocked Khloe Kardashian and Tristan's romance, with the Kardashian removing her diamond ring on Instagram.
Now Tristan has taken legal action over the rumours swirling about his love life.
No Jumper's podcast host Adam John Grandmaison has edited the original interview where model Sydney Chase said she had got into bed with the basketball star.
The podcast star admitted he would never have posted the video if he thought it was going to "mess up a relationship".
He told Page Six : “Yeah, we got a cease-and-desist and deleted it.
“Honestly, I wouldn’t have posted it in the first place if I knew it was going to mess up a relationship.”
Lawyer Marty Singer, acting on behalf of Tristan, confirmed to the publication he had sent the letter to both No Jumper podcast and to Sydney.
In a letter obtained by TMZ, the attorney called Sydney's allegations "malicious defamatory fabrications".
The Mirror approached Tristan Thompson's representatives for further comment.
It comes as Khloe revealed she and Tristan were talking about having baby number two via surrogate in the latest Keeping Up With The Kardashians trailer.
The couple reconciled their romance and went public in March after playing happy families in lockdown with their daughter True.
Khloe has started building her dream home by her mum Kris Jenner's plot of land.
Khloe and Tristan have lots of romantic history together and go back to 2016.
She has been devastated when Tristan cheated on her in the past.
In 2016, footage of the sports star kissing another woman was leaked by TMZ.
All was forgiven when Khloe welcomed their baby daughter True and they vowed to focus on their family together.
At the time, she wrote: "Regardless of what Tristan did to me, I was never going to take that moment away from True or Tristan.
"She should not be punished for his actions. He is her father and they both deserve to love one another as deeply as they possibly can."
In 2019, Tristan locked lips with Kylie Jenner's ex-BFF Jordyn Woods at a house party.
The scandal rocked the Kardashian clan to the core and Kylie dumped her BFF.
Tristan and Khloe split shortly afterwards but since then the couple have moved on and given their love another go.
On Keeping Up With The Kardashians, Tristan apologised to Khloe's mum Kris about cheating on her daughter.
He said: "So when I hurt her with all the actions I did, it really affected me a lot because of how much I let you down and our relationship, because you viewed me as a son."