Several drivers and touts belonging to the Embassava Sacco have been arrested by the National Safety and Transport Authority officers.
This is after a 17-year-old boy died when he was ran over by one of their buses after an argument with a tout.
This saw irate citizen torch the matatu as they agitated over the manner in which the matatus are operated.
The conductor immediately escaped on foot, evading the mob that was baying for his blood.
The safety authority said the country wide crackdown aims at restoring sanity and order on the roads while ensuring maximum discipline in the matatu sector.
The authority's Director General George Njao said although several Saccos are colluding to sabotage their efforts, they will not relent until sanity is restored.
"Several PSV operators especially conductors and drivers will spend their weekend in custody. Embassava Sacco is among those affected after their crew were arrested in the ongoing crackdown and have been handed over to the police," said Mr Njao.
He added, "Some PSV operators have pulled their vehicles off the road to evade our traps but we will not relent. The roads have to be safe for all road users".
Njao urged members of the public to further play a key role in identifying and calling out these rogue drivers and touts.
According to him, restoring sanity on the roads is not a reserve of the authority nor the police but a collective responsibility that everyone must actively play their role.
In 2015, NTSA imposed another ban on the Sacco after two of their buses collided killing two people.
Then NTSA boss Francis Mejja said they had met the bus management and agreed Embassava was to blame before they issued the suspension.
"We met all concerned parties and agreed Embassava was to blame in the case. They have almost two weeks to correct several issues that came up in the process," said Mejja.
The then DG said preliminary inspection had established some buses of the company were not road worthy.
